Journal: International Journal of Molecular Sciences
Article Title: Hedyotis diffusa Suppresses Colitis-Associated Colorectal Cancer via Inhibition of the IL-17A-IL-17RA Axis and NF-κB Signaling
doi: 10.3390/ijms27041745
Figure Lengend Snippet: Effects of HD and FA on NF-κB activation, IL-17A production, and granulocyte infiltration in rectal tissues of AOM/DSS-induced CRC mice. Mice were treated with HD (200 mg/kg) or FA (100 mg/kg) for 91 days. Rectal sections were stained with antibody against p65, IL-17A, or CD11b (200× magnification). Scale bar = 100 µm. Representative images are shown. Quantitative analysis of the stained area or cells (%) is displayed below the image. Data are expressed as mean ± SD (n = 8 mice per group). ### p < 0.001 vs. mock group. ** p < 0.01, *** p < 0.001 vs. CRC group.
Article Snippet: Mouse monoclonal antibody against p65 (MAB3026), rabbit polyclonal antibody against IL-17A (bs-1183R), and rabbit monoclonal antibody against CD11b (ab133357) were obtained from Millipore (Temecula, CA, USA), Bioss Antibodies (Woburn, MA, USA), and abcam (Cambridge, UK), respectively.
Techniques: Activation Assay, Staining